A layup in basketball is a two-point shot attempt made by leaping from below, laying the ball up near the basket, and using one hand to bounce it off the backboard and into the basket. The motion and one-handed reach distinguish it from a jump shot. The layup is considered the most basic shot in basketball. When doing a layup, the player lifts the outside foot, or the foot away from the basket.

An undefended layup is usually a high percentage shot. The main obstacle is getting near the rim and avoiding blocks by taller defenders who usually stand near the basket. Common layup strategies are to create spaces, release the ball from a different spot, or use alternate hands. A player able to reach over the rim might choose to perform a more spectacular and higher percentage slam dunk (dropping or throwing the ball from above the rim) instead.